Hotspot

ndhH-rps15

SSC · 125331-125458 · 128 bp

Back to Callianthemum

Pi 0.0164

MarkerSeek score 65.80

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: SSC
length_bp: 128
variable_sites: 2
pi: 0.0164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	AACACCATGCATTGATGGGT	GTCTGCGGAAAATTCTGGGA	45.0	50.0	58.1	58.2	322-334 bp	1.0	80.1
ndhH-rps15_p2	GAGTCGAAGAACACCATGCA	GTCTGCGGAAAATTCTGGGA	50.0	50.0	58.2	58.2	331-343 bp	1.0	78.8
ndhH-rps15_p3	TGAGTCGAAGAACACCATGC	GTCTGCGGAAAATTCTGGGA	50.0	50.0	58.2	58.2	332-344 bp	1.0	78.8
ndhH-rps15_p4	ATGCATTGATGGGTGGTGAG	GTCTGCGGAAAATTCTGGGA	50.0	50.0	58.2	58.2	316-328 bp	1.0	78.7
ndhH-rps15_p5	CATTGATGGGTGGTGAGGAC	GTCTGCGGAAAATTCTGGGA	55.0	50.0	58.2	58.2	313-325 bp	1.0	78.6