Hotspot

rpl32-trnL-UAG

SSC · 116237-116809 · 573 bp

Back to Callianthemum

Pi 0.0371
MarkerSeek score 69.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
573
variable_sites
21
pi
0.0371
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGCAACAAACAAGTAATAAGGTCT AGCGTGTCTACCGATTTCAC 33.3 50.0 57.0 58.0 631-634 bp 1.0 43.9
rpl32-trnL-UAG_p2 TGCAACAAACAAGTAATAAGGTCT CAGCGTGTCTACCGATTTCA 33.3 50.0 57.0 58.0 632-635 bp 1.0 43.8
rpl32-trnL-UAG_p3 GTGCAACAAACAAGTAATAAGGTC AGCGTGTCTACCGATTTCAC 37.5 50.0 57.0 58.0 632-635 bp 1.0 43.6
rpl32-trnL-UAG_p4 GTGCAACAAACAAGTAATAAGGTC CAGCGTGTCTACCGATTTCA 37.5 50.0 57.0 58.0 633-636 bp 1.0 43.6
rpl32-trnL-UAG_p5 GTGCAACAAACAAGTAATAAGGT AGCGTGTCTACCGATTTCAC 34.8 50.0 55.9 58.0 632-635 bp 1.0 43.5