Hotspot

ndhF-rpl32

SSC · 115092-116062 · 971 bp

Back to Callianthemum

Pi 0.0312
MarkerSeek score 66.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
971
variable_sites
30
pi
0.0312
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GAACTGGAAGGGGAACGAAA AGCTTTTTGGATATAGAAGTACGT 50.0 33.3 57.7 56.0 1067-1149 bp 1.0 42.9
ndhF-rpl32_p2 TGGAAGGGGAACGAAAGGTA AGCTTTTTGGATATAGAAGTACGT 50.0 33.3 58.3 56.0 1063-1145 bp 1.0 42.9
ndhF-rpl32_p3 AACTGGAAGGGGAACGAAAG AGCTTTTTGGATATAGAAGTACGT 50.0 33.3 57.7 56.0 1066-1148 bp 1.0 42.9
ndhF-rpl32_p4 GAACTGGAAGGGGAACGAAA AAGCTTTTTGGATATAGAAGTACGT 50.0 32.0 57.7 56.6 1068-1150 bp 1.0 42.9
ndhF-rpl32_p5 GAACTGGAAGGGGAACGAAA AGCTTTTTGGATATAGAAGTACGTT 50.0 32.0 57.7 56.6 1067-1149 bp 1.0 42.9