Hotspot

trnR-ACG-trnN-GUU

IRb · 110894-111423 · 530 bp

Back to Callianthemum

Pi 0.0132

MarkerSeek score 64.90

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 530
variable_sites: 7
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	671 bp	1.0	79.1
trnR-ACG-trnN-GUU_p2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.7	58.0	708 bp	1.0	78.5
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	622 bp	1.0	77.9
trnR-ACG-trnN-GUU_p4	GAACAGATCCAGTGGAGACG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.7	58.2	659 bp	1.0	77.3
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	CGGTCGGCTGTTAACTGATT	55.0	50.0	57.8	58.3	641 bp	1.0	76.9