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psbE-petL

LSC · 67215-68395 · 1181 bp

Back to Callianthemum

Pi 0.0112

MarkerSeek score 64.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1181
variable_sites: 13
pi: 0.0112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGTTAAAGCTGCCAGCAGAA	50.0	45.0	57.9	57.7	1309-1333 bp	1.0	79.6
psbE-petL_p2	ACGAATAACCAACCCGCAAT	AGTTAAAGCTGCCAGCAGAA	45.0	45.0	58.2	57.7	1318-1342 bp	1.0	78.6
psbE-petL_p3	TGCTGACGAATAACCAACCC	AGTTAAAGCTGCCAGCAGAA	50.0	45.0	58.2	57.7	1323-1347 bp	1.0	78.5
psbE-petL_p4	CGAATAACCAACCCGCAATG	AGTTAAAGCTGCCAGCAGAA	50.0	45.0	57.8	57.7	1317-1341 bp	1.0	78.5
psbE-petL_p5	ACCAACCCGCAATGAATAGG	AGTTAAAGCTGCCAGCAGAA	50.0	45.0	58.2	57.7	1311-1335 bp	1.0	78.1