Hotspot

petA-psbJ

LSC · 66022-66452 · 431 bp

Back to Callianthemum

Pi 0.0142

MarkerSeek score 64.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 431
variable_sites: 6
pi: 0.0142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CATCACGTGTCCAAGGTCTT	TGGCTTATAGGCACTGTAGC	50.0	50.0	57.8	57.1	634-640 bp	1.0	73.0
petA-psbJ_p2	CATCACGTGTCCAAGGTCTT	AGGCACTGTAGCTGGTATTC	50.0	50.0	57.8	57.0	626-632 bp	1.0	72.4
petA-psbJ_p3	CATCACGTGTCCAAGGTCTT	GGCACTGTAGCTGGTATTCT	50.0	50.0	57.8	57.0	625-631 bp	1.0	72.4
petA-psbJ_p4	CATCACGTGTCCAAGGTCTT	GCTGGTATTCTTGTGATCGGT	50.0	47.6	57.8	58.1	616-622 bp	1.0	71.6
petA-psbJ_p5	CATCACGTGTCCAAGGTCTT	AGCTGGTATTCTTGTGATCGG	50.0	47.6	57.8	57.8	617-623 bp	1.0	70.8