Hotspot

trnM-CAU-atpE

LSC · 55188-55480 · 293 bp

Back to Callianthemum

Pi 0.028

MarkerSeek score 70.80

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 293
variable_sites: 8
pi: 0.028
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	GGCAGGAGTCATTGGTTCAA	GAGTCGAGGCTATCAATGCA	50.0	50.0	58.1	57.5	357-360 bp	1.0	78.5
trnM-CAU-atpE_p2	GGCAGGAGTCATTGGTTCAA	CGAGTCGAGGCTATCAATGC	50.0	55.0	58.1	58.6	358-361 bp	1.0	77.9
trnM-CAU-atpE_p3	GGCAGGAGTCATTGGTTCAA	GCGAGTCGAGGCTATCAATG	50.0	55.0	58.1	58.6	359-362 bp	1.0	77.9
trnM-CAU-atpE_p4	TTGCTTTCATACGGCAGGAG	GAGTCGAGGCTATCAATGCA	50.0	50.0	58.3	57.5	369-372 bp	1.0	77.0
trnM-CAU-atpE_p5	TTGCTTTCATACGGCAGGAG	CGAGTCGAGGCTATCAATGC	50.0	55.0	58.3	58.6	370-373 bp	1.0	76.4