Hotspot

ndhC-trnV-UAC

LSC · 53086-54270 · 1185 bp

Back to Callianthemum

Pi 0.0119

MarkerSeek score 63.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1185
variable_sites: 14
pi: 0.0119
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CCTTCGTTAATTGGGGCCAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.4	57.8	1328-1331 bp	1.0	75.9
ndhC-trnV-UAC_p2	TAATTGGGGCCAAAACTCCG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.4	57.8	1321-1324 bp	1.0	75.9
ndhC-trnV-UAC_p3	CCTTCGTTAATTGGGGCCAA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.4	57.5	1327-1330 bp	1.0	73.2
ndhC-trnV-UAC_p4	TAATTGGGGCCAAAACTCCG	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.4	57.5	1320-1323 bp	1.0	73.2
ndhC-trnV-UAC_p5	TAATTGGGGCCAAAACTCCG	GTTCGAGTCCGTATAGCCCT	50.0	55.0	58.4	58.7	1309-1312 bp	1.0	72.0