Hotspot

ycf3-trnS-GGA

LSC · 46455-47299 · 845 bp

Back to Callianthemum

Pi 0.0166

MarkerSeek score 68.80

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 845
variable_sites: 14
pi: 0.0166
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	CGGTCAGTAATGCTCGTGAA	47.6	50.0	58.0	58.0	987-1082 bp	1.0	73.0
ycf3-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	47.6	50.0	58.0	58.1	934-1029 bp	1.0	72.4
ycf3-trnS-GGA_p3	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	955-1050 bp	1.0	71.9
ycf3-trnS-GGA_p4	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	47.6	45.0	58.0	57.8	905-1000 bp	1.0	71.6
ycf3-trnS-GGA_p5	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	47.6	50.0	58.0	58.4	935-1030 bp	1.0	70.0