Hotspot

atpF-atpH

LSC · 12610-13069 · 460 bp

Back to Callianthemum

Pi 0.0218

MarkerSeek score 68.80

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 460
variable_sites: 10
pi: 0.0218
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CAGTGACCCAAGGAAACGAA	ATTTATGGACTGGTCGTGGC	50.0	50.0	58.0	58.0	554-555 bp	1.0	81.1
atpF-atpH_p2	TTAAATCCGAAACTCCCGGC	ATTTATGGACTGGTCGTGGC	50.0	50.0	58.3	58.0	581-582 bp	1.0	79.3
atpF-atpH_p3	CAGTGACCCAAGGAAACGAA	TCGTGGCATTAGCGCTTTTA	50.0	45.0	58.0	58.3	541-542 bp	1.0	79.3
atpF-atpH_p4	TTAAATCCGAAACTCCCGGC	TCGTGGCATTAGCGCTTTTA	50.0	45.0	58.3	58.3	568-569 bp	1.0	77.4
atpF-atpH_p5	AGTGACCCAAGGAAACGAAA	ATTTATGGACTGGTCGTGGC	45.0	50.0	57.3	58.0	553-554 bp	1.0	75.7