Hotspot

trnK-UUU-trnQ-UUG

LSC · 4139-6582 · 2444 bp

Back to Callianthemum

Pi 0.0103
MarkerSeek score 63.30
Primer available yes
Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name
trnK-UUU-trnQ-UUG
feature_type
igs
region
LSC
length_bp
2444
variable_sites
25
pi
0.0103
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-trnQ-UUG_p1 AATCCGTCGCTTCATCCAAA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.9 58.1 2548-2562 bp 1.0 79.8
trnK-UUU-trnQ-UUG_p2 CGTCGCTTCATCCAAATGTG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.8 58.1 2544-2558 bp 1.0 79.4
trnK-UUU-trnQ-UUG_p3 AAAGCCGAGTACTCTACCGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 2502-2516 bp 1.0 79.4
trnK-UUU-trnQ-UUG_p4 AAGCCGAGTACTCTACCGTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 2501-2515 bp 1.0 79.4
trnK-UUU-trnQ-UUG_p5 AATCCGTCGCTTCATCCAAA CTCGGAGGTTCGAATCCTTC 45.0 55.0 57.9 57.8 2552-2566 bp 1.0 78.7