Hotspot

ndhK

Genome · 11403-12086 · 684 bp

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Pi 0.00146

MarkerSeek score 59.60

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: Genome
length_bp: 684
variable_sites: 1
pi: 0.00146
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	ATGGCGAAAGGGAGCATTAG	TTTGTACAAAAGGATCCTGT	50.0	35.0	58.0	52.3	777 bp	1.0	44.1
ndhK_p2	GGCGAAAGGGAGCATTAGAA	TTTGTACAAAAGGATCCTGT	50.0	35.0	58.0	52.3	775 bp	1.0	44.1
ndhK_p3	ATGGCGAAAGGGAGCATTAG	TTTTGTACAAAAGGATCCTGT	50.0	33.3	58.0	53.2	778 bp	1.0	44.1
ndhK_p4	GGCGAAAGGGAGCATTAGAA	TTTTGTACAAAAGGATCCTGT	50.0	33.3	58.0	53.2	776 bp	1.0	44.1
ndhK_p5	TTTATGCATGGCGAAAGGGA	TTTGTACAAAAGGATCCTGT	45.0	35.0	57.9	52.3	784 bp	1.0	44.1