Hotspot

atpB

Genome · 7633-9129 · 1497 bp

Back to Callerya

Pi 0.00134

MarkerSeek score 59.50

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: Genome
length_bp: 1497
variable_sites: 2
pi: 0.00134
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	AACCGATCAACTTGCTTTGT	ACAATTCGATTGGGGGTCAG	40.0	50.0	56.2	57.9	1611 bp	1.0	68.4
atpB_p2	AACCGATCAACTTGCTTTGT	CAATTCGATTGGGGGTCAGT	40.0	50.0	56.2	57.9	1610 bp	1.0	68.4
atpB_p3	ACCGATCAACTTGCTTTGTT	ACAATTCGATTGGGGGTCAG	40.0	50.0	56.2	57.9	1610 bp	1.0	68.4
atpB_p4	ACCGATCAACTTGCTTTGTT	CAATTCGATTGGGGGTCAGT	40.0	50.0	56.2	57.9	1609 bp	1.0	68.4
atpB_p5	ACCGATCAACTTGCTTTGTT	TGGGGGTCAGTACACAAAGA	40.0	50.0	56.2	58.2	1600 bp	1.0	67.9