Hotspot

trnL-UAG-rpl32

Genome · 126421-127073 · 653 bp

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Pi 0.00408
MarkerSeek score 60.80
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
Genome
length_bp
653
variable_sites
4
pi
0.00408
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 TCCTAAGAGCAGCGTGTCTA TGGACAGCATTGAAAGCCTT 50.0 45.0 57.9 58.0 773-793 bp 1.0 79.4
trnL-UAG-rpl32_p2 TCCTAAGAGCAGCGTGTCTA TTGGACAGCATTGAAAGCCT 50.0 45.0 57.9 58.0 774-794 bp 1.0 79.4
trnL-UAG-rpl32_p3 AGCAGCGTGTCTACCAATTT TGGACAGCATTGAAAGCCTT 45.0 45.0 57.8 58.0 766-786 bp 1.0 78.9
trnL-UAG-rpl32_p4 AGCAGCGTGTCTACCAATTT TTGGACAGCATTGAAAGCCT 45.0 45.0 57.8 58.0 767-787 bp 1.0 78.9
trnL-UAG-rpl32_p5 CTAAGAGCAGCGTGTCTACC TGGACAGCATTGAAAGCCTT 55.0 45.0 57.8 58.0 771-791 bp 1.0 78.8