Hotspot

trnG-UCC-trnS-GCU

Genome · 55409-56305 · 897 bp

Back to Callerya

Pi 0.0213

MarkerSeek score 70.90

Primer available yes

Label trnG-UCC-trnS-GCU

Feature Metadata

label_name: trnG-UCC-trnS-GCU
feature_type: igs
region: Genome
length_bp: 897
variable_sites: 19
pi: 0.0213
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnS-GCU_p1	TGAATCAAGCAAGGGATCCC	CAATCCGACGCTTTAGTCCA	50.0	50.0	57.6	58.0	1001-1013 bp	1.0	81.9
trnG-UCC-trnS-GCU_p2	TGAATCAAGCAAGGGATCCC	AATCCGACGCTTTAGTCCAC	50.0	50.0	57.6	58.0	1000-1012 bp	1.0	81.9
trnG-UCC-trnS-GCU_p3	TGAATCAAGCAAGGGATCCC	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.6	58.2	1054-1066 bp	1.0	80.7
trnG-UCC-trnS-GCU_p4	TGAATCAAGCAAGGGATCCC	AGCAATCCGACGCTTTAGTC	50.0	50.0	57.6	58.4	1003-1015 bp	1.0	79.1
trnG-UCC-trnS-GCU_p5	TGAATCAAGCAAGGGATCCC	CGACGCTTTAGTCCACTCAG	50.0	55.0	57.6	58.4	996-1008 bp	1.0	78.9