Hotspot

petN-trnC-GCA

Genome · 34149-35277 · 1129 bp

Back to Callerya

Pi 0.00266

MarkerSeek score 60.30

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: Genome
length_bp: 1129
variable_sites: 3
pi: 0.00266
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	TTAAAGCAGCCCAAGCAAGA	TTTCCCCAGTTCAAATCCGG	45.0	50.0	58.0	58.1	1204-1207 bp	1.0	83.5
petN-trnC-GCA_p2	CAGCCCAAGCAAGACTTACT	TTTCCCCAGTTCAAATCCGG	50.0	50.0	57.8	58.1	1198-1201 bp	1.0	82.0
petN-trnC-GCA_p3	TTAAAGCAGCCCAAGCAAGA	GGGGGACTGCAAATCCTTTT	45.0	50.0	58.0	58.4	1222-1225 bp	1.0	81.3
petN-trnC-GCA_p4	AGAGTCCACTTCTTCCCCAT	TTTCCCCAGTTCAAATCCGG	50.0	50.0	57.6	58.1	1258-1261 bp	1.0	80.7
petN-trnC-GCA_p5	CAGCCCAAGCAAGACTTACT	GGGGGACTGCAAATCCTTTT	50.0	50.0	57.8	58.4	1216-1219 bp	1.0	79.8