Hotspot

trnG-UCC-psbZ

Genome · 26061-26872 · 812 bp

Back to Callerya

Pi 0.00123

MarkerSeek score 59.50

Primer available yes

Label trnG-UCC-psbZ

Feature Metadata

label_name: trnG-UCC-psbZ
feature_type: igs
region: Genome
length_bp: 812
variable_sites: 1
pi: 0.00123
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-psbZ_p1	CGTCTTCTCCTTGGCAAAGA	CTTCTCCTGATGGTTGGTCG	50.0	55.0	57.8	58.0	948-1008 bp	1.0	82.6
trnG-UCC-psbZ_p2	TACATAAAAAGGCAGGGGCG	CTTCTCCTGATGGTTGGTCG	50.0	55.0	58.2	58.0	988-1048 bp	1.0	82.0
trnG-UCC-psbZ_p3	CGTCTTCTCCTTGGCAAAGA	TTGCTTCTCCTGATGGTTGG	50.0	50.0	57.8	57.8	951-1011 bp	1.0	81.1
trnG-UCC-psbZ_p4	CGTCTTCTCCTTGGCAAAGA	TCCTGATGGTTGGTCGAGTA	50.0	50.0	57.8	57.8	944-1004 bp	1.0	80.9
trnG-UCC-psbZ_p5	CGTCTTCTCCTTGGCAAAGA	GCTTCTCCTGATGGTTGGTC	50.0	55.0	57.8	58.3	949-1009 bp	1.0	80.7