Hotspot

ycf3-psaA

Genome · 19715-20677 · 963 bp

Back to Callerya

Pi 0.00208

MarkerSeek score 59.90

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: Genome
length_bp: 963
variable_sites: 2
pi: 0.00208
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	AGCCCTTACCCCTGGTAATT	TGCCCATTCCTCGAAAGAAG	50.0	50.0	58.0	57.9	1109-1111 bp	1.0	83.2
ycf3-psaA_p2	AGCCCTTACCCCTGGTAATT	GCCCATTCCTCGAAAGAAGT	50.0	50.0	58.0	57.9	1108-1110 bp	1.0	83.2
ycf3-psaA_p3	AGCTATAGCCCTTACCCCTG	TGCCCATTCCTCGAAAGAAG	55.0	50.0	58.0	57.9	1115-1117 bp	1.0	83.0
ycf3-psaA_p4	AGCTATAGCCCTTACCCCTG	GCCCATTCCTCGAAAGAAGT	55.0	50.0	58.0	57.9	1114-1116 bp	1.0	83.0
ycf3-psaA_p5	TTGGTTGAAGATCACAGGGC	TGCCCATTCCTCGAAAGAAG	50.0	50.0	58.1	57.9	1073-1075 bp	1.0	82.5