Hotspot

ndhJ-trnF-GAA

Genome · 12713-13451 · 739 bp

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Pi 0.00271

MarkerSeek score 60.30

Primer available yes

Label ndhJ-trnF-GAA

Feature Metadata

label_name: ndhJ-trnF-GAA
feature_type: igs
region: Genome
length_bp: 739
variable_sites: 2
pi: 0.00271
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-trnF-GAA_p1	TTGGATAGGATGGCCTTTGC	GGGATAGCTCAGCTGGTAGA	50.0	55.0	57.9	58.0	879 bp	1.0	83.6
ndhJ-trnF-GAA_p2	TTGGATAGGATGGCCTTTGC	CCTCGTGTCACCAGTTCAAA	50.0	50.0	57.9	58.1	843 bp	1.0	83.2
ndhJ-trnF-GAA_p3	TTGGATAGGATGGCCTTTGC	AGAATGGTCGGGATAGCTCA	50.0	50.0	57.9	57.9	888 bp	1.0	82.9
ndhJ-trnF-GAA_p4	TTGGATAGGATGGCCTTTGC	CTGGTAGAGCAGAGGACTGA	50.0	55.0	57.9	57.9	867 bp	1.0	82.5
ndhJ-trnF-GAA_p5	TTGGATAGGATGGCCTTTGC	AGTTCAAATCTGGTTCCCGG	50.0	50.0	57.9	57.8	831 bp	1.0	82.0