Hotspot

rbcL-atpB

Genome · 6885-7632 · 748 bp

Back to Callerya

Pi 0.00134

MarkerSeek score 59.50

Primer available yes

Label rbcL-atpB

Feature Metadata

label_name: rbcL-atpB
feature_type: igs
region: Genome
length_bp: 748
variable_sites: 1
pi: 0.00134
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-atpB_p1	AACACCAGCTTTGAACCCAA	AGCTACATCTAGGACCGGAC	45.0	55.0	58.1	58.0	900-903 bp	1.0	82.8
rbcL-atpB_p2	AACACCAGCTTTGAACCCAA	CCTGGGGGAAAAGCTACATC	45.0	55.0	58.1	57.9	911-914 bp	1.0	82.5
rbcL-atpB_p3	AACACCAGCTTTGAACCCAA	CGGACCGATTATTTGGACGA	45.0	50.0	58.1	57.8	885-888 bp	1.0	81.3
rbcL-atpB_p4	GCTTTGAACCCAACACTTGC	AGCTACATCTAGGACCGGAC	50.0	55.0	58.4	58.0	893-896 bp	1.0	80.5
rbcL-atpB_p5	AACACCAGCTTTGAACCCAA	ACCGGACCGATTATTTGGAC	45.0	50.0	58.1	57.7	887-890 bp	1.0	80.4