Hotspot

psbA-trnH-GUG

LSC · 82739-83143 · 405 bp

Back to Calendula

Pi 0.00758
MarkerSeek score 61.20
Primer available yes
Label psbA-trnH-GUG

Feature Metadata

label_name
psbA-trnH-GUG
feature_type
igs
region
LSC
length_bp
405
variable_sites
3
pi
0.00758
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnH-GUG_p1 CCGTGCTAACCTTGGTATGG GATCCACTTGGCTACATCCG 55.0 55.0 58.3 58.1 515-524 bp 1.0 78.1
psbA-trnH-GUG_p2 CCGTGCTAACCTTGGTATGG CAATCCACTGCCTTGATCCA 55.0 50.0 58.3 57.9 529-538 bp 1.0 78.0
psbA-trnH-GUG_p3 CCGTGCTAACCTTGGTATGG AATCCACTGCCTTGATCCAC 55.0 50.0 58.3 57.9 528-537 bp 1.0 78.0
psbA-trnH-GUG_p4 CCGTGCTAACCTTGGTATGG ACAATCCACTGCCTTGATCC 55.0 50.0 58.3 57.9 530-539 bp 1.0 78.0
psbA-trnH-GUG_p5 ACCGTGCTAACCTTGGTATG GATCCACTTGGCTACATCCG 50.0 55.0 57.6 58.1 516-525 bp 1.0 77.6