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atpH-atpI

LSC · 57386-58413 · 1028 bp

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Pi 0.00486

MarkerSeek score 61.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1028
variable_sites: 5
pi: 0.00486
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACATTAGCCGCAG	45.0	45.0	58.0	57.9	1115 bp	1.0	79.5
atpH-atpI_p2	CAGTACCTTGACCAACTCCG	TTTTGCAACATTAGCCGCAG	55.0	45.0	57.9	57.9	1174 bp	1.0	79.2
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	TTTTGCAACATTAGCCGCAG	45.0	45.0	57.9	57.9	1131 bp	1.0	78.7
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	TTTTGCAACATTAGCCGCAG	45.0	45.0	58.2	57.9	1116 bp	1.0	78.4
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	TTTTGCAACATTAGCCGCAG	55.0	45.0	58.2	57.9	1175 bp	1.0	78.3