Hotspot

trnT-GGU-trnG-UCC

LSC · 52486-52656 · 171 bp

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Pi 0.012
MarkerSeek score 62.20
Primer available yes
Label trnT-GGU-trnG-UCC

Feature Metadata

label_name
trnT-GGU-trnG-UCC
feature_type
igs
region
LSC
length_bp
171
variable_sites
2
pi
0.012
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnG-UCC_p1 GATTGGAACCGAGGGCTTAC ATCAAACCGAAAGACCCCTT 55.0 45.0 58.3 57.0 307-312 bp 1.0 71.4
trnT-GGU-trnG-UCC_p2 GATTGGAACCGAGGGCTTAC AATCAAACCGAAAGACCCCT 55.0 45.0 58.3 57.0 308-313 bp 1.0 71.3
trnT-GGU-trnG-UCC_p3 GCCGCTTATTGGATTGGAAC AATCAAACCGAAAGACCCCT 50.0 45.0 57.5 57.0 319-324 bp 1.0 70.0
trnT-GGU-trnG-UCC_p4 GCCGCTTATTGGATTGGAAC ATCAAACCGAAAGACCCCTT 50.0 45.0 57.5 57.0 318-323 bp 1.0 70.0
trnT-GGU-trnG-UCC_p5 GATTGGAACCGAGGGCTTAC GAATCAAACCGAAAGACCCCT 55.0 47.6 58.3 58.2 309-314 bp 1.0 69.4