Hotspot

ndhJ-trnF-GAA

LSC · 35141-35814 · 674 bp

Back to Calendula

Pi 0.00568

MarkerSeek score 62.00

Primer available yes

Label ndhJ-trnF-GAA

Feature Metadata

label_name: ndhJ-trnF-GAA
feature_type: igs
region: LSC
length_bp: 674
variable_sites: 3
pi: 0.00568
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-trnF-GAA_p1	AGTTGGATAGGATGGCCCTT	GTGCAGAGACTCGATGGAAG	50.0	55.0	58.1	58.1	1306-1452 bp	1.0	82.9
ndhJ-trnF-GAA_p2	ACAAGTTTCAGGTTGGGCAT	GTGCAGAGACTCGATGGAAG	45.0	55.0	57.9	58.1	1961-2107 bp	1.0	82.8
ndhJ-trnF-GAA_p3	GAAAAAGTCGCGGCTCAATC	GTGCAGAGACTCGATGGAAG	50.0	55.0	58.1	58.1	1792-1938 bp	1.0	82.6
ndhJ-trnF-GAA_p4	TGGCATTCCATTGCTGTCAT	GTGCAGAGACTCGATGGAAG	45.0	55.0	58.1	58.1	1606-1752 bp	1.0	82.4
ndhJ-trnF-GAA_p5	ACAAGTTTCAGGTTGGGCAT	AGAGACTCGATGGAAGCTGT	45.0	50.0	57.9	58.2	1957-2103 bp	1.0	82.2