Hotspot

rbcL-atpB

LSC · 28749-29483 · 735 bp

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Pi 0.00409

MarkerSeek score 61.20

Primer available yes

Label rbcL-atpB

Feature Metadata

label_name: rbcL-atpB
feature_type: igs
region: LSC
length_bp: 735
variable_sites: 3
pi: 0.00409
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-atpB_p1	CACCAGCTTTGAATCCAACAC	CTACATCTAGTACCGGCCCA	47.6	55.0	58.0	58.0	877-878 bp	1.0	72.2
rbcL-atpB_p2	ACCAGCTTTGAATCCAACACT	CTACATCTAGTACCGGCCCA	42.9	55.0	58.1	58.0	876-877 bp	1.0	71.9
rbcL-atpB_p3	CACCAGCTTTGAATCCAACAC	ATCTAGTACCGGCCCAATGA	47.6	50.0	58.0	57.9	873-874 bp	1.0	71.5
rbcL-atpB_p4	CACCAGCTTTGAATCCAACAC	TCTAGTACCGGCCCAATGAT	47.6	50.0	58.0	57.9	872-873 bp	1.0	71.5
rbcL-atpB_p5	CACCAGCTTTGAATCCAACAC	GATACGCCCCAGGTTCTTTT	47.6	50.0	58.0	57.9	846-847 bp	1.0	71.2