primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps12-rpl20_p1	rps12-rpl20	1	CAGCGTCGAGGAACATGTAC	TCTCCGAGCGATATATCCCC	20	20	55.0	55.0	58.7	57.9	9.8	7.1	0.785066	14312	15044	799-799	1.000000	74.1
rps12-rpl20_p2	rps12-rpl20	2	AGCGTCGAGGAACATGTACT	TCTCCGAGCGATATATCCCC	20	20	50.0	55.0	58.8	57.9	9.8	7.1	0.879449	14312	15044	798-798	1.000000	73.4
rps12-rpl20_p3	rps12-rpl20	3	TCAGCGTCGAGGAACATGTA	TCTCCGAGCGATATATCCCC	20	20	50.0	55.0	58.8	57.9	9.8	7.1	0.883790	14312	15044	800-800	1.000000	73.3
rps12-rpl20_p4	rps12-rpl20	4	GCGTCGAGGAACATGTACTA	TCTCCGAGCGATATATCCCC	20	20	50.0	55.0	57.2	57.9	9.8	7.1	0.892618	14312	15044	797-797	1.000000	73.3
rps12-rpl20_p5	rps12-rpl20	5	CAGCGTCGAGGAACATGTAC	TTCTACGTCTCCGAGCGATA	20	20	55.0	50.0	58.7	57.8	9.8	15.9	0.960509	14312	15044	806-806	1.000000	72.7
petG-petL_p1	petG-petL	1	TCAAAGGTCCAACTGATCGC	TAGCTCCTTGATGCCTACCA	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.366275	17768	17932	385-385	1.000000	77.7
petG-petL_p2	petG-petL	2	TCAAAGGTCCAACTGATCGC	AGCTCCTTGATGCCTACCAT	20	20	50.0	50.0	58.2	58.5	0.0	0.0	0.675480	17768	17932	384-384	1.000000	75.2
petG-petL_p3	petG-petL	3	CGCCACGCCTGTATTGTAAA	TAGCTCCTTGATGCCTACCA	20	20	50.0	50.0	58.9	57.8	0.0	0.0	1.094387	17768	17932	368-368	1.000000	71.9
petG-petL_p4	petG-petL	4	TCAAAGGTCCAACTGATCGC	TGGTCTGAGCAAGATACGAC	20	20	50.0	50.0	58.2	57.1	0.0	7.1	1.133042	17768	17932	307-307	1.000000	71.7
petG-petL_p5	petG-petL	5	TCAAAGGTCCAACTGATCGC	AGCTCCTTGATGCCTACCA	20	19	50.0	52.6	58.2	58.0	0.0	0.0	1.203742	17768	17932	384-384	1.000000	71.0
psbJ-petA_p1	psbJ-petA	1	TGTGATCGGTTTAGTAGGCG	ACGTGTACAAGGCCTTTTGT	20	20	50.0	45.0	57.4	58.0	0.0	17.6	0.619192	20068	20851	953-960	1.000000	76.0
psbJ-petA_p2	psbJ-petA	2	GTGATCGGTTTAGTAGGCGT	ACGTGTACAAGGCCTTTTGT	20	20	50.0	45.0	57.4	58.0	0.0	17.6	0.620943	20068	20851	952-959	1.000000	76.0
psbJ-petA_p3	psbJ-petA	3	TGTGATCGGTTTAGTAGGCG	GGCCTTTTGTTCTTCTTCGC	20	20	50.0	50.0	57.4	57.9	0.0	0.0	0.624069	20068	20851	943-950	1.000000	75.9
psbJ-petA_p4	psbJ-petA	4	GTGATCGGTTTAGTAGGCGT	GGCCTTTTGTTCTTCTTCGC	20	20	50.0	50.0	57.4	57.9	0.0	0.0	0.625820	20068	20851	942-949	1.000000	75.9
psbJ-petA_p5	psbJ-petA	5	TATTCCGGATTGGGTTCGTC	ACGTGTACAAGGCCTTTTGT	20	20	50.0	45.0	57.4	58.0	4.1	17.6	0.654633	20068	20851	916-923	1.000000	75.7
rbcL-atpB_p1	rbcL-atpB	1	CACCAGCTTTGAATCCAACAC	CTACATCTAGTACCGGCCCA	21	20	47.6	55.0	58.0	58.0	0.0	0.0	1.026144	28749	29483	877-878	1.000000	72.2
rbcL-atpB_p2	rbcL-atpB	2	ACCAGCTTTGAATCCAACACT	CTACATCTAGTACCGGCCCA	21	20	42.9	55.0	58.1	58.0	0.0	0.0	1.059432	28749	29483	876-877	1.000000	71.9
rbcL-atpB_p3	rbcL-atpB	3	CACCAGCTTTGAATCCAACAC	ATCTAGTACCGGCCCAATGA	21	20	47.6	50.0	58.0	57.9	0.0	0.0	1.114375	28749	29483	873-874	1.000000	71.5
rbcL-atpB_p4	rbcL-atpB	4	CACCAGCTTTGAATCCAACAC	TCTAGTACCGGCCCAATGAT	21	20	47.6	50.0	58.0	57.9	0.0	0.0	1.114375	28749	29483	872-873	1.000000	71.5
rbcL-atpB_p5	rbcL-atpB	5	CACCAGCTTTGAATCCAACAC	GATACGCCCCAGGTTCTTTT	21	20	47.6	50.0	58.0	57.9	0.0	0.0	1.141542	28749	29483	846-847	1.000000	71.2
trnV-UAC-ndhC_p1	trnV-UAC-ndhC	1	AGAAGGTCTACGGTTCGAGT	GTCCTTTGCTAATCGGAGCT	20	20	50.0	50.0	57.8	57.7	0.0	0.0	0.514813	32476	33464	1134-1154	1.000000	76.6
trnV-UAC-ndhC_p2	trnV-UAC-ndhC	2	AGAAGGTCTACGGTTCGAGT	CGGTCCTTTGCTAATCGGAG	20	20	50.0	55.0	57.8	58.4	0.0	0.0	0.625364	32476	33464	1136-1156	1.000000	75.7
trnV-UAC-ndhC_p3	trnV-UAC-ndhC	3	AGAAGGTCTACGGTTCGAGT	CTCCGGTCCTTTGCTAATCG	20	20	50.0	55.0	57.8	58.4	0.0	0.0	0.625364	32476	33464	1139-1159	1.000000	75.7
trnV-UAC-ndhC_p4	trnV-UAC-ndhC	4	GAAGGTCTACGGTTCGAGTC	GTCCTTTGCTAATCGGAGCT	20	20	55.0	50.0	57.5	57.7	0.0	0.0	0.858585	32476	33464	1133-1153	1.000000	73.8
trnV-UAC-ndhC_p5	trnV-UAC-ndhC	5	AGAAGGTCTACGGTTCGAGT	GGTCCTTTGCTAATCGGAGC	20	20	50.0	55.0	57.8	58.7	0.0	3.4	0.896802	32476	33464	1135-1155	1.000000	73.5
ndhJ-trnF-GAA_p1	ndhJ-trnF-GAA	1	AGTTGGATAGGATGGCCCTT	GTGCAGAGACTCGATGGAAG	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.167220	35141	35814	1306-1452	1.000000	82.9
ndhJ-trnF-GAA_p2	ndhJ-trnF-GAA	2	ACAAGTTTCAGGTTGGGCAT	GTGCAGAGACTCGATGGAAG	20	20	45.0	55.0	57.9	58.1	0.0	0.0	0.166692	35141	35814	1961-2107	1.000000	82.8
ndhJ-trnF-GAA_p3	ndhJ-trnF-GAA	3	GAAAAAGTCGCGGCTCAATC	GTGCAGAGACTCGATGGAAG	20	20	50.0	55.0	58.1	58.1	16.4	0.0	0.196606	35141	35814	1792-1938	1.000000	82.6
ndhJ-trnF-GAA_p4	ndhJ-trnF-GAA	4	TGGCATTCCATTGCTGTCAT	GTGCAGAGACTCGATGGAAG	20	20	45.0	55.0	58.1	58.1	0.0	0.0	0.224787	35141	35814	1606-1752	1.000000	82.4
ndhJ-trnF-GAA_p5	ndhJ-trnF-GAA	5	ACAAGTTTCAGGTTGGGCAT	AGAGACTCGATGGAAGCTGT	20	20	45.0	50.0	57.9	58.2	0.0	0.0	0.240604	35141	35814	1957-2103	1.000000	82.2
trnL-UAA-trnT-UGU_p1	trnL-UAA-trnT-UGU	1	ACCAATTTCGCCATATCCCC	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.137364	36696	37225	576-580	1.000000	80.0
trnL-UAA-trnT-UGU_p2	trnL-UAA-trnT-UGU	2	TAGCGTCTACCAATTTCGCC	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.202173	36696	37225	584-588	1.000000	79.5
trnL-UAA-trnT-UGU_p3	trnL-UAA-trnT-UGU	3	ACCAATTTCGCCATATCCCC	GGAATCGAACCGATGACCAT	20	20	50.0	50.0	58.0	57.8	0.0	15.5	0.245117	36696	37225	608-612	1.000000	79.1
trnL-UAA-trnT-UGU_p4	trnL-UAA-trnT-UGU	4	TAGCGTCTACCAATTTCGCC	GGAATCGAACCGATGACCAT	20	20	50.0	50.0	58.1	57.8	0.0	15.5	0.309926	36696	37225	616-620	1.000000	78.6
trnL-UAA-trnT-UGU_p5	trnL-UAA-trnT-UGU	5	AGTCCGTAGCGTCTACCAAT	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.2	57.9	10.2	0.0	0.372548	36696	37225	590-594	1.000000	78.1
trnT-GGU-trnG-UCC_p1	trnT-GGU-trnG-UCC	1	GATTGGAACCGAGGGCTTAC	ATCAAACCGAAAGACCCCTT	20	20	55.0	45.0	58.3	57.0	0.0	0.0	1.307027	52486	52656	307-312	1.000000	71.4
trnT-GGU-trnG-UCC_p2	trnT-GGU-trnG-UCC	2	GATTGGAACCGAGGGCTTAC	AATCAAACCGAAAGACCCCT	20	20	55.0	45.0	58.3	57.0	0.0	0.0	1.307027	52486	52656	308-313	1.000000	71.3
trnT-GGU-trnG-UCC_p3	trnT-GGU-trnG-UCC	3	GCCGCTTATTGGATTGGAAC	AATCAAACCGAAAGACCCCT	20	20	50.0	45.0	57.5	57.0	0.0	0.0	1.463920	52486	52656	319-324	1.000000	70.0
trnT-GGU-trnG-UCC_p4	trnT-GGU-trnG-UCC	4	GCCGCTTATTGGATTGGAAC	ATCAAACCGAAAGACCCCTT	20	20	50.0	45.0	57.5	57.0	0.0	0.0	1.463920	52486	52656	318-323	1.000000	70.0
trnT-GGU-trnG-UCC_p5	trnT-GGU-trnG-UCC	5	GATTGGAACCGAGGGCTTAC	GAATCAAACCGAAAGACCCCT	20	21	55.0	47.6	58.3	58.2	0.0	0.0	1.543472	52486	52656	309-314	1.000000	69.4
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACATTAGCCGCAG	20	20	45.0	45.0	58.0	57.9	1.4	0.0	0.101543	57386	58413	1115-1115	1.000000	79.5
atpH-atpI_p2	atpH-atpI	2	CAGTACCTTGACCAACTCCG	TTTTGCAACATTAGCCGCAG	20	20	55.0	45.0	57.9	57.9	0.0	0.0	0.143442	57386	58413	1174-1174	1.000000	79.2
atpH-atpI_p3	atpH-atpI	3	AGCCAATCCAGCAGCAATAA	TTTTGCAACATTAGCCGCAG	20	20	45.0	45.0	57.9	57.9	0.0	0.0	0.211601	57386	58413	1131-1131	1.000000	78.7
atpH-atpI_p4	atpH-atpI	4	AATAACGGAAGCGGCAGAAA	TTTTGCAACATTAGCCGCAG	20	20	45.0	45.0	58.2	57.9	1.4	0.0	0.247081	57386	58413	1116-1116	1.000000	78.4
atpH-atpI_p5	atpH-atpI	5	GCAGTACCTTGACCAACTCC	TTTTGCAACATTAGCCGCAG	20	20	55.0	45.0	58.2	57.9	0.0	0.0	0.253472	57386	58413	1175-1175	1.000000	78.3
rpoB-trnE-UUC_p1	rpoB-trnE-UUC	1	AGTTCCTCTGTCAAACCTTGA	GGGACTTGAACCCTCGTTTT	21	20	42.9	50.0	56.8	58.0	0.0	0.0	2.237066	70574	71500	1069-1069	1.000000	62.8
rpoB-trnE-UUC_p2	rpoB-trnE-UUC	2	AGTTCCTCTGTCAAACCTTGA	GAGTTTTGGTACACCCCCAG	21	20	42.9	55.0	56.8	58.1	0.0	0.0	2.308485	70574	71500	1090-1090	1.000000	62.2
rpoB-trnE-UUC_p3	rpoB-trnE-UUC	3	AGTTCCTCTGTCAAACCTTGA	GTTTTGGTACACCCCCAGAG	21	20	42.9	55.0	56.8	58.1	0.0	0.0	2.308485	70574	71500	1088-1088	1.000000	62.2
rpoB-trnE-UUC_p4	rpoB-trnE-UUC	4	AGTTCCTCTGTCAAACCTTGA	TAACCACTGGACCATAGGGG	21	20	42.9	55.0	56.8	58.1	0.0	14.8	2.326325	70574	71500	1028-1028	1.000000	62.1
rpoB-trnE-UUC_p5	rpoB-trnE-UUC	5	AGTTCCTCTGTCAAACCTTGA	CCCTCGTTTTCTCCGTGAAA	21	20	42.9	50.0	56.8	58.1	0.0	0.0	2.340425	70574	71500	1059-1059	1.000000	62.0
trnD-GUC-psbM_p1	trnD-GUC-psbM	1	TTCAATAGGCAAGAGCACCG	AGTAGAAAGGCAGTAGGAACT	20	21	50.0	42.9	58.3	55.7	0.0	0.0	3.550437	72004	72633	762-762	1.000000	52.0
trnD-GUC-psbM_p2	trnD-GUC-psbM	2	TTCAATAGGCAAGAGCACCG	GGAACTAAAATGAACAGTGCAGT	20	23	50.0	39.1	58.3	57.6	0.0	0.0	3.700923	72004	72633	747-747	1.000000	50.8
trnD-GUC-psbM_p3	trnD-GUC-psbM	3	TTCAATAGGCAAGAGCACCG	AAAATGAACAGTGCAGTAGC	20	20	50.0	40.0	58.3	54.5	0.0	0.0	3.756773	72004	72633	741-741	1.000000	50.4
trnD-GUC-psbM_p4	trnD-GUC-psbM	4	TTCAATAGGCAAGAGCACCG	AAGTAGAAAGGCAGTAGGAACT	20	22	50.0	40.9	58.3	56.5	0.0	0.0	3.786412	72004	72633	763-763	1.000000	50.1
trnD-GUC-psbM_p5	trnD-GUC-psbM	5	TTCAATAGGCAAGAGCACCG	ACTAAAATGAACAGTGCAGTAGC	20	23	50.0	39.1	58.3	57.4	0.0	0.0	3.875520	72004	72633	744-744	1.000000	49.4
trnQ-UUG-rps16_p1	trnQ-UUG-rps16	1	GAGGTTCGAATCCTTCCGTC	AAGTCGCACGTTGCTTTCTA	20	20	55.0	45.0	58.1	58.1	16.9	8.1	0.215425	76211	77059	909-922	1.000000	80.1
trnQ-UUG-rps16_p2	trnQ-UUG-rps16	2	GAGGTTCGAATCCTTCCGTC	TCATGTCCTTCAAGTCGCAC	20	20	55.0	50.0	58.1	58.2	16.9	0.0	0.280333	76211	77059	920-933	1.000000	79.6
trnQ-UUG-rps16_p3	trnQ-UUG-rps16	3	GAGGTTCGAATCCTTCCGTC	CACACCGGATCATGTCCTTC	20	20	55.0	55.0	58.1	58.3	16.9	9.5	0.419449	76211	77059	929-942	1.000000	78.4
trnQ-UUG-rps16_p4	trnQ-UUG-rps16	4	GAGGTTCGAATCCTTCCGTC	CACCGGATCATGTCCTTCAA	20	20	55.0	50.0	58.1	57.6	16.9	9.5	0.477684	76211	77059	927-940	1.000000	78.0
trnQ-UUG-rps16_p5	trnQ-UUG-rps16	5	GAGGTTCGAATCCTTCCGTC	CCGGATCATGTCCTTCAAGT	20	20	55.0	50.0	58.1	57.3	16.9	0.0	0.774956	76211	77059	925-938	1.000000	75.6
psbA-trnH-GUG_p1	psbA-trnH-GUG	1	CCGTGCTAACCTTGGTATGG	GATCCACTTGGCTACATCCG	20	20	55.0	55.0	58.3	58.1	0.0	0.0	0.465225	82739	83143	515-524	1.000000	78.1
psbA-trnH-GUG_p2	psbA-trnH-GUG	2	CCGTGCTAACCTTGGTATGG	CAATCCACTGCCTTGATCCA	20	20	55.0	50.0	58.3	57.9	0.0	0.0	0.469587	82739	83143	529-538	1.000000	78.0
psbA-trnH-GUG_p3	psbA-trnH-GUG	3	CCGTGCTAACCTTGGTATGG	AATCCACTGCCTTGATCCAC	20	20	55.0	50.0	58.3	57.9	0.0	0.0	0.471094	82739	83143	528-537	1.000000	78.0
psbA-trnH-GUG_p4	psbA-trnH-GUG	4	CCGTGCTAACCTTGGTATGG	ACAATCCACTGCCTTGATCC	20	20	55.0	50.0	58.3	57.9	0.0	0.0	0.471094	82739	83143	530-539	1.000000	78.0
psbA-trnH-GUG_p5	psbA-trnH-GUG	5	ACCGTGCTAACCTTGGTATG	GATCCACTTGGCTACATCCG	20	20	50.0	55.0	57.6	58.1	0.0	0.0	0.525510	82739	83143	516-525	1.000000	77.6
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TATAGGGCGGCCTTAAAAGC	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	57.7	58.0	37.4	0.0	0.273419	111242	111926	807-807	1.000000	78.2
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TATAGGGCGGCCTTAAAAGC	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	57.7	58.0	37.4	0.0	0.274790	111242	111926	808-808	1.000000	78.2
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GCGGCCTTAAAAGCTTTGTC	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.3	58.0	13.5	0.0	0.309665	111242	111926	801-801	1.000000	77.9
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GCGGCCTTAAAAGCTTTGTC	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.3	58.0	13.5	0.0	0.311036	111242	111926	802-802	1.000000	77.9
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGGATATAGGGCGGCCTTAA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	57.7	58.0	37.4	0.0	0.345189	111242	111926	811-811	1.000000	77.6
ndhD_p1	ndhD	1	GCCGCTCGACTCTTCAAAAT	GGAAATAGGGTAATCCGGTGG	20	21	50.0	52.4	58.9	57.9	0.0	0.0	2.056506	113363	114745	1468-1468	1.000000	63.7
ndhD_p2	ndhD	2	CCGCTCGACTCTTCAAAATC	GGAAATAGGGTAATCCGGTGG	20	21	50.0	52.4	56.9	57.9	0.0	0.0	2.211190	113363	114745	1467-1467	1.000000	62.5
ndhD_p3	ndhD	3	GCCGCTCGACTCTTCAAAAT	AGGGTAATCCGGTGGTATACT	20	21	50.0	47.6	58.9	57.4	0.0	0.0	2.545749	113363	114745	1462-1462	1.000000	59.8
ndhD_p4	ndhD	4	TTTTTCATTCGCCGCTCGA	GGAAATAGGGTAATCCGGTGG	19	21	47.4	52.4	58.5	57.9	0.0	0.0	2.601557	113363	114745	1478-1478	1.000000	59.3
ndhD_p5	ndhD	5	GCCGCTCGACTCTTCAAAA	GGAAATAGGGTAATCCGGTGG	19	21	52.6	52.4	58.5	57.9	0.0	0.0	2.607212	113363	114745	1468-1468	1.000000	59.3
rps15-trnN-GUU_p1	rps15-trnN-GUU	1	TCTTGTTCTCCTTGCAAAAGA	GAATCTACCCGTCTCTCTGT	21	20	38.1	50.0	55.6	55.8	0.0	0.0	5.570444	121734	121883	307-307	1.000000	41.0
rps15-trnN-GUU_p2	rps15-trnN-GUU	2	TCTTGTTCTCCTTGCAAAAGAA	GAATCTACCCGTCTCTCTGT	22	20	36.4	50.0	56.4	55.8	16.2	0.0	5.813882	121734	121883	307-307	1.000000	41.0
rps15-trnN-GUU_p3	rps15-trnN-GUU	3	ATCTTGTTCTCCTTGCAAAAGA	GAATCTACCCGTCTCTCTGT	22	20	36.4	50.0	56.2	55.8	9.3	0.0	6.026185	121734	121883	308-308	1.000000	41.0
rps15-trnN-GUU_p4	rps15-trnN-GUU	4	CTTGTTCTCCTTGCAAAAGA	GAATCTACCCGTCTCTCTGT	20	20	40.0	50.0	54.0	55.8	0.0	0.0	6.180942	121734	121883	306-306	1.000000	41.0
rps15-trnN-GUU_p5	rps15-trnN-GUU	5	TCTTGTTCTCCTTGCAAAAG	GAATCTACCCGTCTCTCTGT	20	20	40.0	50.0	54.0	55.8	0.0	0.0	6.180942	121734	121883	307-307	1.000000	41.0
ycf15-rps12_p1	ycf15-rps12	1	GAAAGGACTCACTAAGCCGG	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	58.0	57.7	0.0	16.5	0.269231	136014	136958	1020-1040	1.000000	78.1
ycf15-rps12_p2	ycf15-rps12	2	CGGTACAATCCTCTTCCTGC	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	58.1	57.7	0.0	16.5	0.316366	136014	136958	1107-1127	1.000000	77.7
ycf15-rps12_p3	ycf15-rps12	3	GTACAATCCTCTTCCTGCCG	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	58.1	57.7	0.0	16.5	0.316366	136014	136958	1105-1125	1.000000	77.7
ycf15-rps12_p4	ycf15-rps12	4	TACAATCCTCTTCCTGCCGA	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	58.1	57.7	0.0	16.5	0.397050	136014	136958	1104-1124	1.000000	77.0
ycf15-rps12_p5	ycf15-rps12	5	GGTACAATCCTCTTCCTGCC	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	57.7	57.7	0.0	16.5	0.591379	136014	136958	1106-1126	1.000000	75.5