Hotspot

rps19

IRb · 90730-91667 · 938 bp

Back to Caldesia

Pi 0.11

MarkerSeek score 78.90

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: IRb
length_bp: 938
variable_sites: 103
pi: 0.11
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	CCCCACACCCATAGATGATG	TGATGGTCAGAGGCGAATTG	55.0	50.0	57.4	58.0	1922 bp	0.5	55.2
rps19_p2	CCCCACACCCATAGATGATG	TGTTTGAAGCACGGACAGAA	55.0	45.0	57.4	58.0	1987 bp	0.5	55.2
rps19_p3	CCCCACACCCATAGATGATG	TTGTTTGAAGCACGGACAGA	55.0	45.0	57.4	58.0	1988 bp	0.5	55.2
rps19_p4	CCCCACACCCATAGATGATG	ATGAGCGATTCGGCAGATTT	55.0	45.0	57.4	58.0	1730 bp	0.5	55.1
rps19_p5	CCCCACACCCATAGATGATG	CCTACCTTTGAGTGCGGTTT	55.0	50.0	57.4	58.1	2209 bp	0.5	54.5