Hotspot

ndhK

LSC · 54028-54771 · 744 bp

Back to Caldesia

Pi 0.00672

MarkerSeek score 62.60

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 744
variable_sites: 5
pi: 0.00672
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	CCCCCTTTGTATTTCGATGGA	GGTTCAGTTTATGCATGGCG	47.6	50.0	57.7	57.8	899 bp	1.0	68.6
ndhK_p2	CCCCCTTTGTATTTCGATGGA	TGGGCGATGAGTTTTGATGT	47.6	45.0	57.7	57.8	974 bp	1.0	68.5
ndhK_p3	CCCCCTTTGTATTTCGATGGA	GTTTATGCATGGCGAAAGGG	47.6	50.0	57.7	57.8	893 bp	1.0	68.5
ndhK_p4	CCCCCTTTGTATTTCGATGGA	ATCCATGGGCGATGAGTTTT	47.6	45.0	57.7	57.6	979 bp	1.0	66.5
ndhK_p5	CCCCCTTTGTATTTCGATGGA	CCATGGGCGATGAGTTTTGA	47.6	50.0	57.7	58.5	977 bp	1.0	65.8