Hotspot

ndhA

SSC · 135389-137584 · 2196 bp

Back to Caldesia

Pi 0.00507

MarkerSeek score 47.90

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2196
variable_sites: 11
pi: 0.00507
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TTCAAAATGAATTCGGCCGC	CAATGTTACGAGCTTCGGGA	45.0	50.0	58.0	58.0	2949-2975 bp	1.0	81.1
ndhA_p2	TTCAAAATGAATTCGGCCGC	TGTGAGAGTAGAAGCCCCAA	45.0	50.0	58.0	58.0	2633-2659 bp	1.0	81.0
ndhA_p3	ACTGACATTGGTAATCGGCC	TGTGAGAGTAGAAGCCCCAA	50.0	50.0	58.0	58.0	2899-2925 bp	1.0	80.7
ndhA_p4	GTTGGGCAATACTCCACACA	TGTGAGAGTAGAAGCCCCAA	50.0	50.0	58.1	58.0	2800-2826 bp	1.0	80.3
ndhA_p5	TGTTGGGCAATACTCCACAC	TGTGAGAGTAGAAGCCCCAA	50.0	50.0	58.1	58.0	2801-2827 bp	1.0	80.3