Hotspot

ccsA

SSC · 129702-130670 · 969 bp

Back to Caldesia

Pi 0.00516

MarkerSeek score 53.70

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 5
pi: 0.00516
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGGCGGCATCATTCTAGAA	GGTGAGAGTGAGGTAGAGGG	50.0	60.0	58.0	58.2	1130 bp	1.0	78.1
ccsA_p2	GAGTGGCGGCATCATTCTAG	GGTGAGAGTGAGGTAGAGGG	55.0	60.0	58.5	58.2	1132 bp	1.0	74.6
ccsA_p3	GTGGCGGCATCATTCTAGAA	GTGAGAGTGAGGTAGAGGGA	50.0	55.0	58.0	57.2	1129 bp	1.0	73.5
ccsA_p4	GTGGCGGCATCATTCTAGAA	AGGTGAGAGTGAGGTAGAGG	50.0	55.0	58.0	57.2	1131 bp	1.0	73.4
ccsA_p5	AGTGGCGGCATCATTCTAGA	GGTGAGAGTGAGGTAGAGGG	50.0	60.0	58.9	58.2	1131 bp	1.0	71.5