Hotspot

rpl22-rps19

LSC · 89969-90729 · 761 bp

Back to Caldesia

Pi 0.205

MarkerSeek score 82.80

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 761
variable_sites: 150
pi: 0.205
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TTTCTTCATAGGAACGCCCG	CGTTTTATTCTTCGTCGCCG	50.0	50.0	58.0	57.9	1280 bp	0.5	59.4
rpl22-rps19_p2	TAACTACGTCCTCTAGCCCG	CGTTTTATTCTTCGTCGCCG	55.0	50.0	58.0	57.9	1467 bp	0.5	59.2
rpl22-rps19_p3	TTTCTTCATAGGAACGCCCG	AAAAGTTTGGGTAGAGCCGG	50.0	50.0	58.0	58.1	1475 bp	0.5	59.1
rpl22-rps19_p4	CCTCTAGCCCGAGGTCTTAA	CGTTTTATTCTTCGTCGCCG	55.0	50.0	57.9	57.9	1458 bp	0.5	59.0
rpl22-rps19_p5	TTTCTTCATAGGAACGCCCG	TGGGGAGGTCCGTTTGATAT	50.0	50.0	58.0	58.1	1548 bp	0.5	59.0