Hotspot

psaJ-rpl33

LSC · 72833-73278 · 446 bp

Back to Caldesia

Pi 0.0615

MarkerSeek score 83.20

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 446
variable_sites: 27
pi: 0.0615
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TATCTCTCTGTGGCACCTGT	GGCACAACTGGTACATTCCA	50.0	50.0	57.8	58.1	614-616 bp	1.0	81.8
psaJ-rpl33_p2	TGGTTTGGGTCTTTAGCAGG	GGCACAACTGGTACATTCCA	50.0	50.0	57.7	58.1	581-583 bp	1.0	81.6
psaJ-rpl33_p3	GGTTTGGGTCTTTAGCAGGT	GGCACAACTGGTACATTCCA	50.0	50.0	57.7	58.1	580-582 bp	1.0	81.6
psaJ-rpl33_p4	TATCTCTCTGTGGCACCTGT	TTATGAGTGTCCTTTCGGGC	50.0	50.0	57.8	57.6	631-633 bp	1.0	79.2
psaJ-rpl33_p5	TGGTTTGGGTCTTTAGCAGG	TTATGAGTGTCCTTTCGGGC	50.0	50.0	57.7	57.6	598-600 bp	1.0	79.1