Hotspot

petG-trnW-CCA

LSC · 71858-71961 · 104 bp

Back to Caldesia

Pi 0.0577

MarkerSeek score 82.20

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 104
variable_sites: 6
pi: 0.0577
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	GACGTGGTGATCAGTTGGAT	AAGCGCTCTTAGTTCAGCTC	50.0	50.0	57.6	58.0	206 bp	1.0	79.2
petG-trnW-CCA_p2	GACGTGGTGATCAGTTGGAT	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.6	57.9	169 bp	1.0	79.0
petG-trnW-CCA_p3	AGACGTGGTGATCAGTTGGA	AAGCGCTCTTAGTTCAGCTC	50.0	50.0	58.7	58.0	207 bp	1.0	77.0
petG-trnW-CCA_p4	AGACGTGGTGATCAGTTGGA	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.7	57.9	170 bp	1.0	76.8
petG-trnW-CCA_p5	GACGTGGTGATCAGTTGGAT	CCCGATGTCGTAGGTTCAAA	50.0	50.0	57.6	57.6	164 bp	1.0	76.8