Hotspot

accD-psaI

LSC · 64475-65442 · 968 bp

Back to Caldesia

Pi 0.0093

MarkerSeek score 59.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 968
variable_sites: 9
pi: 0.0093
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	ACAAGCATCCGAGTGTTTGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	1156-1176 bp	1.0	80.1
accD-psaI_p2	AGCATCCGAGTGTTTGTACC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	1153-1173 bp	1.0	79.6
accD-psaI_p3	ACAAGCATCCGAGTGTTTGT	AAGAAGCCATTGCAATTGCC	45.0	45.0	58.0	57.9	1162-1182 bp	1.0	79.5
accD-psaI_p4	AGCATCCGAGTGTTTGTACC	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.9	57.9	1159-1179 bp	1.0	79.0
accD-psaI_p5	GCATCCGAGTGTTTGTACCA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	1152-1172 bp	1.0	78.8