Hotspot

psaA-ycf3

LSC · 45750-46306 · 557 bp

Back to Caldesia

Pi 0.0054

MarkerSeek score 61.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 557
variable_sites: 3
pi: 0.0054
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	TGGTTGAAGATTACAGGGCG	50.0	50.0	57.9	57.9	665-666 bp	1.0	78.9
psaA-ycf3_p2	TTGAGAAATGGCCTGGTCTG	TGGTTGAAGATTACAGGGCG	50.0	50.0	57.8	57.9	686-687 bp	1.0	78.0
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	TGGTTTGATCAAGCTGCTGA	50.0	45.0	57.9	57.7	740-741 bp	1.0	77.5
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	GCTATAGCGCTTACTCCAGG	50.0	55.0	57.9	57.6	707-708 bp	1.0	76.8
psaA-ycf3_p5	TTGAGAAATGGCCTGGTCTG	TGGTTTGATCAAGCTGCTGA	50.0	45.0	57.8	57.7	761-762 bp	1.0	76.6