Hotspot

psbZ-trnG-UCC

LSC · 39582-40416 · 835 bp

Back to Caldesia

Pi 0.00845

MarkerSeek score 64.40

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 835
variable_sites: 7
pi: 0.00845
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	TTTGCTTCTCCTGACGGTTG	CGGATAGCGGGAATCGAAC	50.0	57.9	58.4	58.2	1008-1015 bp	1.0	68.3
psbZ-trnG-UCC_p2	TTTGCTTCTCCTGACGGTTG	GATAGCGGGAATCGAACCC	50.0	57.9	58.4	57.8	1006-1013 bp	1.0	68.2
psbZ-trnG-UCC_p3	TTTGCTTCTCCTGACGGTTG	GGATAGCGGGAATCGAACC	50.0	57.9	58.4	57.8	1007-1014 bp	1.0	68.2
psbZ-trnG-UCC_p4	ATTTGCTTCTCCTGACGGTT	CGGATAGCGGGAATCGAAC	45.0	57.9	57.4	58.2	1009-1016 bp	1.0	67.0
psbZ-trnG-UCC_p5	ATTTGCTTCTCCTGACGGTT	GATAGCGGGAATCGAACCC	45.0	57.9	57.4	57.8	1007-1014 bp	1.0	66.9