Hotspot

rps16-trnQ-UUG

LSC · 6504-8097 · 1594 bp

Back to Caldesia

Pi 0.0108
MarkerSeek score 57.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1594
variable_sites
17
pi
0.0108
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA TGGTCCTGTTATTCGGAGGT 45.0 50.0 58.1 58.1 1657-1682 bp 1.0 80.5
rps16-trnQ-UUG_p2 TGATGTCCTTCAAGTCGCAC TGGTCCTGTTATTCGGAGGT 50.0 50.0 58.2 58.1 1668-1693 bp 1.0 79.9
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1646-1671 bp 1.0 75.6
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA TAAGGCAACCGGTTTTGGTC 45.0 50.0 58.1 58.7 1672-1697 bp 1.0 75.4
rps16-trnQ-UUG_p5 ATGATGTCCTTCAAGTCGCA TGGTCCTGTTATTCGGAGGT 45.0 50.0 57.2 58.1 1669-1694 bp 1.0 75.4