Hotspot

trnH-GUG-psbA

LSC · 114-405 · 292 bp

Back to Caldesia

Pi 0.0205
MarkerSeek score 62.10
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
292
variable_sites
6
pi
0.0205
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG GCGCTAACCTTGGTATGGAA 55.0 50.0 58.1 58.0 409-426 bp 1.0 79.9
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCTGT 55.0 55.0 58.1 57.9 359-376 bp 1.0 79.7
trnH-GUG-psbA_p3 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 362-379 bp 1.0 79.7
trnH-GUG-psbA_p4 GATCCACTTGGCTACATCCG ATCGCGCTAACCTTGGTATG 55.0 50.0 58.1 58.1 412-429 bp 1.0 79.0
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG CTGTTGAAGCTCCATCCACA 55.0 50.0 58.1 57.8 343-360 bp 1.0 78.8