Hotspot

ndhF-rpl32

SSC · 115523-116541 · 1019 bp

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Pi 0.0251
MarkerSeek score 69.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1019
variable_sites
160
pi
0.0251
unique_haplotype_count
31
primer_available
yes
alignment_reliability
0.95
markerseek_score
69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAAAAGGGGTATTATCCATGC AGAAAAAGCTTTTACTGCCTCT 43.5 36.4 57.5 56.2 829-1258 bp 1.0 52.0
ndhF-rpl32_p2 GGAAAAAGGGGTATTATCCATGC AAGAAAAAGCTTTTACTGCCTCT 43.5 34.8 57.5 56.9 830-1259 bp 1.0 52.0
ndhF-rpl32_p3 GGAAAAAGGGGTATTATCCATGC GAAAAAGCTTTTACTGCCTCT 43.5 38.1 57.5 54.7 828-1257 bp 1.0 52.0
ndhF-rpl32_p4 GGAAAAAGGGGTATTATCCATGC GCTTTTACTGCCTCTAAAGATCC 43.5 43.5 57.5 57.3 822-1251 bp 0.973684 50.9
ndhF-rpl32_p5 AAAGGGGTATTATCCATGCA GCTTTTACTGCCTCTAAAGATCC 40.0 43.5 54.0 57.3 818-1247 bp 0.973684 50.9