Hotspot

trnR-ACG-trnN-GUU

IRb · 111377-111894 · 518 bp

Back to Calanthe

Pi 0.00748

MarkerSeek score 64.60

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 518
variable_sites: 35
pi: 0.00748
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	621-665 bp	1.0	86.8
trnR-ACG-trnN-GUU_p2	TTTGAAGGTACTCCCGGGAA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	58.3	58.0	675-719 bp	1.0	86.0
trnR-ACG-trnN-GUU_p3	TTTTGAAGGTACTCCCGGGA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	58.3	58.0	676-720 bp	1.0	86.0
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	CGGTTGGCTGTTAACTGACT	55.0	50.0	57.8	58.1	591-635 bp	1.0	86.0
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	GCTGTTAACTGACTGGTCGT	55.0	50.0	57.8	57.8	585-629 bp	1.0	85.7