Hotspot

rps12-trnV-GAC

IRb · 102283-103521 · 1239 bp

Back to Calanthe

Pi 0.013

MarkerSeek score 68.20

Primer available yes

Label rps12-trnV-GAC

Feature Metadata

label_name: rps12-trnV-GAC
feature_type: igs
region: IRb
length_bp: 1239
variable_sites: 68
pi: 0.013
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-trnV-GAC_p1	TGGCAACTTTACGTAAGGCA	GATGACTTCCACCACGTCAA	45.0	50.0	57.7	57.8	1335-1609 bp	1.0	84.2
rps12-trnV-GAC_p2	TGGCAACTTTACGTAAGGCA	AACTGATGACTTCCACCACG	45.0	50.0	57.7	57.8	1339-1613 bp	1.0	84.2
rps12-trnV-GAC_p3	TGGCAACTTTACGTAAGGCA	ATGACTTCCACCACGTCAAG	45.0	50.0	57.7	57.8	1334-1608 bp	1.0	84.2
rps12-trnV-GAC_p4	TGGCAACTTTACGTAAGGCA	CTGATGACTTCCACCACGTC	45.0	55.0	57.7	58.3	1337-1611 bp	1.0	83.3
rps12-trnV-GAC_p5	TGGCAACTTTACGTAAGGCA	GTCAAGGTGACACTCTACCG	45.0	55.0	57.7	57.7	1320-1594 bp	1.0	82.8