Hotspot

clpP-psbB

LSC · 73985-74784 · 800 bp

Back to Calanthe

Pi 0.0221

MarkerSeek score 66.60

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 800
variable_sites: 91
pi: 0.0221
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	728-1128 bp	1.0	90.1
clpP-psbB_p2	TATACGTCAACCCAAGCTGC	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	723-1123 bp	1.0	90.0
clpP-psbB_p3	CTTTTGGAACACCAATGGGC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.8	58.2	674-1074 bp	1.0	88.8
clpP-psbB_p4	CGCACTATACGTCAACCCAA	ACACGATACCAAGGCAAACC	50.0	50.0	58.0	58.5	731-1131 bp	1.0	88.2
clpP-psbB_p5	TCCTCTCCAGGATTCCGAAA	CGATACCAAGGCAAACCCAT	50.0	50.0	57.8	58.2	699-1099 bp	1.0	88.1