Hotspot

psaJ-rpl33

LSC · 68759-69316 · 558 bp

Back to Calanthe

Pi 0.021

MarkerSeek score 64.70

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 558
variable_sites: 60
pi: 0.021
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	AAAACAAACGCGCTACCAAG	ATGCCCGGCGATTCTTTATT	45.0	45.0	57.9	58.0	1159-1200 bp	1.0	89.7
psaJ-rpl33_p2	CTCAACGGCACCTGTGATAA	AATGCCCGGCGATTCTTTAT	50.0	45.0	57.9	58.0	754-761 bp	0.236842	50.2
psaJ-rpl33_p3	CTCAACGGCACCTGTGATAA	ATGCCCGGCGATTCTTTATT	50.0	45.0	57.9	58.0	753-760 bp	0.236842	50.2
psaJ-rpl33_p4	CGAGGGGTCTTGCGTATATG	AATGCCCGGCGATTCTTTAT	55.0	45.0	57.9	58.0	1331 bp	0.105263	44.0
psaJ-rpl33_p5	CGAGGGGTCTTGCGTATATG	ATGCCCGGCGATTCTTTATT	55.0	45.0	57.9	58.0	1330 bp	0.105263	44.0