Hotspot

atpB-rbcL

LSC · 55587-56560 · 974 bp

Back to Calanthe

Pi 0.0285

MarkerSeek score 70.10

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 974
variable_sites: 132
pi: 0.0285
unique_haplotype_count: 33
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TGCCCGGTGGAAAAATAACA	GTCCACACAGTTGTCCATGT	45.0	50.0	58.0	58.0	1284-1383 bp	1.0	91.7
atpB-rbcL_p2	TGCCCGGTGGAAAAATAACA	AGAAACGATCTCTCCAACGC	45.0	50.0	58.0	58.0	1727-1826 bp	1.0	90.7
atpB-rbcL_p3	TGCCCGGTGGAAAAATAACA	CTTTAAAAAGCGCTTCGGCA	45.0	45.0	58.0	58.0	1754-1853 bp	1.0	90.3
atpB-rbcL_p4	AGACTAGGGCCACTTTGGAT	AGAAACGATCTCTCCAACGC	50.0	50.0	58.0	58.0	2319-2417 bp	0.842105	82.4
atpB-rbcL_p5	TGCCCGGTGGAAAAATAACA	TCGGCTTGCGCTTTAAAAAG	45.0	45.0	58.0	58.0	1777-1863 bp	0.578947	70.2