Hotspot

rps16-trnQ-UUG

LSC · 6107-7220 · 1114 bp

Back to Calanthe

Pi 0.0467

MarkerSeek score 85.70

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1114
variable_sites: 237
pi: 0.0467
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 85.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	ATCCACAGCCAATCATGTCC	GACCAAATCAAAATGGGGCG	50.0	50.0	57.9	58.0	1083-1444 bp	1.0	91.4
rps16-trnQ-UUG_p2	ATCCACAGCCAATCATGTCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1022-1383 bp	1.0	90.9
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GACCAAATCAAAATGGGGCG	45.0	50.0	58.1	58.0	1060-1421 bp	1.0	90.8
rps16-trnQ-UUG_p4	CCACAGCCAATCATGTCCTT	GACCAAATCAAAATGGGGCG	50.0	50.0	58.2	58.0	1081-1442 bp	1.0	90.7
rps16-trnQ-UUG_p5	TCATGTCCTTCAAGTCGCAC	GACCAAATCAAAATGGGGCG	50.0	50.0	58.2	58.0	1071-1432 bp	1.0	90.3