Hotspot

clpP

LSC · 71172-73293 · 2122 bp

Back to Calamus

Pi 0.00529

MarkerSeek score 57.20

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2122
variable_sites: 29
pi: 0.00529
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGGAATGGATTGGTTTAGATCGA	AACCCATTGTTACGTTTCCA	39.1	40.0	57.6	55.5	2218-2298 bp	1.0	44.2
clpP_p2	TGGAATGGATTGGTTTAGATCGA	AAACCCATTGTTACGTTTCCA	39.1	38.1	57.6	56.3	2219-2299 bp	1.0	44.2
clpP_p3	TGGAATGGATTGGTTTAGATCGA	ACCCATTGTTACGTTTCCAT	39.1	40.0	57.6	55.2	2217-2297 bp	1.0	44.2
clpP_p4	TGGAATGGATTGGTTTAGATCG	AACCCATTGTTACGTTTCCA	40.9	40.0	56.2	55.5	2218-2298 bp	1.0	44.2
clpP_p5	TGGAATGGATTGGTTTAGATCGA	AACCCATTGTTACGTTTCCAT	39.1	38.1	57.6	56.0	2218-2298 bp	1.0	44.2