Hotspot

rps15-ycf1

SSC · 125582-125901 · 320 bp

Back to Calamus

Pi 0.0154

MarkerSeek score 47.70

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 320
variable_sites: 11
pi: 0.0154
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CAAGAGCCGTTGACGTTTTC	TGTAACCTTCCCCTGGCATA	50.0	50.0	58.0	58.0	391-550 bp	1.0	85.6
rps15-ycf1_p2	CAAGAGCCGTTGACGTTTTC	ACGGCAATATGCTTGTGGAT	50.0	45.0	58.0	57.9	758-917 bp	1.0	84.8
rps15-ycf1_p3	AACACCATGCATTGATGGGT	TGTAACCTTCCCCTGGCATA	45.0	50.0	58.1	58.0	662-821 bp	1.0	84.7
rps15-ycf1_p4	TCTTGTAGCCGGTACAGTCA	TGTAACCTTCCCCTGGCATA	50.0	50.0	58.1	58.0	608-767 bp	1.0	84.6
rps15-ycf1_p5	AACACCATGCATTGATGGGT	ACGGCAATATGCTTGTGGAT	45.0	45.0	58.1	57.9	1029-1188 bp	1.0	84.3