Hotspot

ndhF-rpl32

SSC · 114783-115127 · 345 bp

Back to Calamus

Pi 0.0168

MarkerSeek score 56.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 345
variable_sites: 13
pi: 0.0168
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CATACGTGGAGGGGGAATTG	TCTACATACCACAGCGGCTA	55.0	50.0	58.0	57.9	1372-1400 bp	1.0	81.2
ndhF-rpl32_p2	CATACGTGGAGGGGGAATTG	TTCGGGCTACGTTAATTCCG	55.0	50.0	58.0	58.1	1280-1308 bp	1.0	81.1
ndhF-rpl32_p3	CATACGTGGAGGGGGAATTG	TACCACAGCGGCTAGTTCTA	55.0	50.0	58.0	57.9	1366-1394 bp	1.0	80.6
ndhF-rpl32_p4	AGCAACTGCACCGACAAATA	TCTACATACCACAGCGGCTA	45.0	50.0	58.1	57.9	1343-1371 bp	1.0	80.6
ndhF-rpl32_p5	TAGCAACTGCACCGACAAAT	TCTACATACCACAGCGGCTA	45.0	50.0	58.1	57.9	1344-1372 bp	1.0	80.6