Hotspot

petA-psbJ

LSC · 63928-64938 · 1011 bp

Back to Calamus

Pi 0.00659

MarkerSeek score 53.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1011
variable_sites: 16
pi: 0.00659
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GCAATCCCAATGTAGGAGGG	TGCGGGTTGGTTATTTGTCA	55.0	45.0	58.0	58.0	1376-1869 bp	1.0	84.6
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	TGCGGGTTGGTTATTTGTCA	50.0	45.0	58.0	58.0	1322-1815 bp	1.0	84.3
petA-psbJ_p3	AGTTGATGCATCGGATGGAC	TGCGGGTTGGTTATTTGTCA	50.0	45.0	58.0	58.0	1480-1973 bp	1.0	84.3
petA-psbJ_p4	GCATCGGATGGACATCAAGT	TGCGGGTTGGTTATTTGTCA	50.0	45.0	58.0	58.0	1473-1966 bp	1.0	84.3
petA-psbJ_p5	TGCATCGGATGGACATCAAG	TGCGGGTTGGTTATTTGTCA	50.0	45.0	58.0	58.0	1474-1967 bp	1.0	84.3