Hotspot

trnC-GCA-petN

LSC · 28026-29121 · 1096 bp

Back to Calamus

Pi 0.00803

MarkerSeek score 61.00

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1096
variable_sites: 22
pi: 0.00803
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGGGACTGCAAATCCTTTA	TAAAGCAGCCCAAGTGAGAC	50.0	50.0	57.5	57.8	935-1202 bp	1.0	79.9
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTA	ACCATTAAAGCAGCCCAAGT	50.0	45.0	57.5	57.7	940-1207 bp	1.0	78.9
trnC-GCA-petN_p3	CCAGTTCAAATCCGGGTGTC	TAAAGCAGCCCAAGTGAGAC	55.0	50.0	58.8	57.8	912-1179 bp	1.0	77.4
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTA	GCAGCCCAAGTGAGACTTAC	50.0	55.0	57.5	58.6	931-1198 bp	1.0	76.9
trnC-GCA-petN_p5	CCAGTTCAAATCCGGGTGTC	ACCATTAAAGCAGCCCAAGT	55.0	45.0	58.8	57.7	917-1184 bp	1.0	76.4